Ann. Méd. Vét., 2002, 146 (1), pp 31 - 38 Isolation and characterization of equine osteocalcin.CARSTANJEN B., WATTIEZ R., AMORY H., LEPAGE O.M., REMY B.Abstract :
Equine bone gamma-carboxyglutamic acid containing protein - also called osteocalcin - has been isolated for the first time till homogeneity. After acid demineralisation and acid extraction, the purification procedure was based on reverse phase chromatography, gel filtration and ion exchange chromatography. The amino acid sequence of the primary structure has been determined to be YLDHWLGA(HYP)APYPDPL(GLA)PRR(GLA)VC (GLA)LNPdCDELADHIGFQeayrrfygpv. The protein has a calculated molecular weight of 5732,36 Da and a calculated isoelectric point of 4,45. It contains 49 amino acids, from which 3 are gamma-carboxyglutamic acid residues at position 17, 21 and 24 and has a fully hydroxylated proline in position nine and cysteine in position 23 and 29. Equine osteocalcin shows a strong homology with bovine (98 %), ovine (96 %) and human (94 %) osteocalcin. Get the PDF Contact person : bremy@ulg.ac.be |