Ann. Méd. Vét., 2014, 158 (1), pp 57-72 Equine semen freezing: state of the art and perspectivesPONTHIER J., VAN DEN BERGHE F., PARRILLA-HERNANDEZ S., HANZEN C., DELEUZE S.Abstract :
Aim of this paper is to review
equine semen freezing procedures.
Spermatozoa formation
inside the testis, spermatozoa
anatomy and physiology will be
summarized, highlighting structures
and/or functions impaired
during freezing/thawing process.
New freezing procedures will be
detailed and discussed. Current
quality standards will be defined
for fresh and thawed semen with
a special attention on total and
progressive motilities and different
definitions of these parameters
in literature. New ways of semen
analysis (such as flow cytometry)
will be discussed for their scientific
and clinical implications. Future
developments and progress in
freezing methods will be exposed,
such as prediction of post-thaw
semen quality on fresh semen
basis, improvement of freezing
extenders and prevention of reactive
oxygen species effects. Get the PDF Contact person : Jerome.Ponthier@ulg.ac.be |