Ann. Méd. Vét., 2005, 149 (3), pp 166-172 Identification by real time polymerization chain reaction of polymorphisms of PrP gene in two Belgian herds declared positive for transmissible spongiform encephalopatiesRENARD C., FONTAINE S., CUVELIER P., MULLENDER C., ROELS S.Abstract :
Following the emergence of the transmissible spongiform encephalopathies (TSEs), several measures were taken : on the one hand, an active detection based on the European regulation 999/2001 and on the other hand, a selection plan of sheep “resistant” to scrapie. These measures increased the request for genotyping of PrP gene for sensitivity to scrapie in sheep. Therefore, it was necessary to develop techniques that are faster and easier to implement than those classically used for routine genotyping. Although more expensive, the technique of real time polymerization chain reaction offers an interesting alternative to the traditional techniques (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism, Denaturing Gradient Gel Electrophoresis...) as well on the level of repeatability, reproducibility and robustness. The analysis of the genetic profiles of individuals composing two herds declared positive for TSE within the framework of the program of active monitoring, highlights an important presence (75 %) of the profile which corresponds to an average resistance to scrapie. The selection of resistant animals seems to be justified since the animals found positive have a genotype of rather low resistance. Get the PDF Contact person : coren@var.fgov.be |