Ann. Méd. Vét., 2005, 149 (3), pp 161-165 Genotypic frequencies of the ryanodin receptor gene mutation associated to stress sensibility in 5 pig production systems in BelgiumCHINA B., LEROY B., DAMS L., CLINQUART A., DAUBE G.Abstract :
Porcine stress syndrome is related to a point mutation in Ryr1 gene encoding the ryanodin receptor. This syndrome involves mortality after stress conditions or poor quality meat. Five hundred and five individuals among 5 Belgian production systems were genotyped. This genotyping was performed by polymerase chain reaction-restriction fragment length polymoprhism (PCR-RFLP) or real time PCR. The results indicated that 22,4 % of the pigs were stress sensitive (TT genotype) and 77,6 % stress resistant (63,4 % CT heterozygotes and 14,2 % CC genotype). Statistical analysis allowed to split the production systems into two groups: the production system 1 and 2 presenting a high level of TT individuals (44 % and 52 % respectively) although production systems 3, 4 and 5 presented a high level of heterozygotes individuals (74 %, 92 % and 78 % respectively). At the technical level, real time PCR appeared to be a faster and an easier technique than PCRRFLP. Get the PDF Contact person : bchina@ulg.ac.be |